Cyto-gcamp6f

WebCyto-GCaMP6f expression when driven by Aldh1l1- Cre/ERT2 in Ai95 mice at ~P66 was dependent on the tamoxifen dose administered in vivo. A. Representative images showing cyto-GCaMP6f expression as a function of tamoxifen dose in hippocampal CA1, V1 visual cortex and striatal brain sections. B. WebDec 3, 2024 · Consistent with the high efficiency and specificity of GCaMP6f labeling in our previous studies (Qin et al., 2024; Zhang et al., 2024), the microinjection of this virus resulted in reliable and...

New Transgenic Mouse Lines for Selectively Targeting …

WebDec 16, 2024 · Cyto-GCaMP6f fluorescence is localized to Müller glia and a subset of neurons. Scale bar: 10 µm. Right, average projection of IPL in Slc1a3-CreER;Lck-GCaMP6f flox retinas at P10 following incubation in vehicle or 200 µM BAPTA-AM. Lck-GCaMP6f fluorescence is localized to Müller glial processes. GCaMP is a genetically encoded calcium indicator (GECI) initially developed in 2001 by Junichi Nakai. It is a synthetic fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light-chain kinase. When bound to Ca , GCaMP fluoresces green with a peak excitation wavelength of 480 nm and a peak emission wavelength of 510 nm. It is used in biological … small car polishing machine https://grupobcd.net

Astrocytes in the mouse visual cortex reliably respond to visual ...

WebDec 16, 2024 · Cyto-GCaMP6f fluorescence is localized to Müller glia and a subset of neurons. Scale bar: 10 µm. Right, average projection of IPL in Slc1a3-CreER;Lck … WebSep 21, 2024 · (A and B) Cre −/− and Cre +/− macrophages were transduced Cyto-GCaMP6f (A) or Mito-GCaMP6f (B) and then incubated with CellVue Claret-labeled ACs. Confocal microscopy was used to capture time-lapse images every 5 min, with measurements beginning when an AC fell into the plane of focus of a macrophage in the … WebJan 7, 2024 · product information Catalog Number : 52925 Product Name : pZac2.1 gfaABC1D-cyto-GCaMP6f article : bacterial resistance : Ampicillin cloning : growth strain : Fluorescent reporter for calcium signaling origin : 37 pi : resistance markers : 753 tags : High Copy more info or order : Addgene product webpage company information Addgene somerset county library nj hours

029626 - R26-Lck-GCaMP6f[flox] Strain Details - The Jackson …

Category:pZac2.1 gfaABC1D-cyto-GCaMP6f Addgene 52925 product …

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Cyto-gcamp6f

Mitochondrial Fission Promotes the Continued Clearance of

WebPurpose. Ready-to-use AAV5 particles produced from pZac2.1 gfaABC1D-lck-GCaMP6f (#52924). In addition to the viral particles, you will also receive purified pZac2.1 … WebPlasmid pGP-CMV-GCaMP6f from Dr. Douglas Kim's lab contains the insert GCaMP6f and is published in Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354. This …

Cyto-gcamp6f

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WebDec 21, 2016 · Cyto-GCaMP6f and Lck-GCaMP6f expression was sparse with Slc1a3-Cre/ERT mice, but expression of both GECIs was ubiquitous and high with Aldh1l1 … WebJul 11, 2024 · Virus & Injection Primary Virus pZac2.1 gfaABC1D-cyto-GCaMP6f (AAV5) Promoter GFAP Injected Titer 5.00E+12 GC/ml Injected Volume 1μl Injection Rate 0.07 μl/min

WebLck-GCaMP6f revealed entire bushy astrocytes, whereas cyto-GCaMP6f revealed the somata, major branches and branchlets (Supp Figure 3 A,B; n = 4 mice). Moreover, with live slice imaging we could... WebJul 11, 2024 · AAV2/5-gfaABC1D-cyto-GCaMP6f (Addgene 52925); viral titer 6.13 × 10 13. 5. Stereotaxic apparatus . 6. Microinjector. 7. Needle/glass micropipettes. 8. Isoflurane. 9. Scalpels. 10. Surgical drill. 11. Heat blanket. 12. Mouse body temperature probe. 2.2 Optical Fiber Implantations 1.

WebSep 30, 2024 · Two 300 nl injections (600 nl total virus) of AAV5-GFaABC1D.cyto-GCaMP6f were made into visual cortex (0.5–1.0 mm anterior and 1.75–2.5 mm lateral of bregma) at a depth of 0.2–0.3 mm and 0.5 ... WebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

WebJul 29, 2024 · pZac2.1 gfaABC1D-cyto-GCaMP6f (AAV5) Promoter GFAP Injected Titer 3.60E+13 GC/ml Injected Volume 30 nl/site, 8 sites per animal, 240 nl total Injection Rate …

WebJul 11, 2024 · pZac2.1 gfaABC1D-cyto-GCaMP6f in Mouse (AAV5) Addgene #52925-AAV5. Caption:Olympus Confocal microscopy, 488nm laser, XLPLN25XWMP2. Data … somerset county library paWebLck-GCaMP6f is a detector of single neuronal action potentials with fast response kinetics. It has an Lck-tag to improve its ability to reveal calcium signals in near membrane regions … small car price philippinesWebReady-to-use AAV5 particles produced from pZac2.1 gfaABC1D-cyto-GCaMP6f (#52925). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-cyto … Overview of Viral Plasmids. While plasmids are typically used to encode, propagate, … Comprehensive molecular biology blog covering topics such as plasmids, viral … small car ports under $500WebBased on immunohistochemical analysis, GCaMP6f expression was almost colocalized with the astrocyte marker s100β (Figures 1C,E) and not detected within neurons (Figures 1D,F) in either the cortex... somerset county library marylandWebIt was clear that Lck-GCaMP6f and cyto-GCaMP6f were expressed in the vast majority of S100β positive astrocytes in the hippocampus, visual cortex and striatum, with occasional patches of S100β... somerset county maine probationWebSep 1, 2024 · A craniotomy was made to expose the visual cortex, and AAV2/5-gfaABC1D-cyto-GCaMP6f was injected to transduce cortical astrocytes for visualization of astrocytic Ca 2+ signals. Two-photon imaging of basal astrocyte Ca 2+ activity was conducted through a cranial window, 3 to 4 weeks later. somerset county maine administrator\u0027s officeWebIn addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression … small carport for motorcycle