WebPolyphosphates (polyP) are polymers of inorganic phosphates joined by high-energy bonds to form long chains. These chains are present in all forms of life but were once disregarded as ‘molecular... WebHere we report a direct fluorescence based DAPI assay system which removes the requirement for prior polyP extraction before quantification. This increased the efficiency …

Direct quantification of inorganic polyphosphate in microbial cells ...

WebDAPI (4′,6-diamidino-2-phenylindole), a widely used fluorescent label for DNA, also interacts with polyphosphate. Binding of poly-P to DAPI, shifts its peak emission wavelength … WebWhen overstained with DAPI (long exposure or high concentration), polyphosphate granules were visible as yel- low fluorescence (Fig. 1E), while nuclear staining faded. No overstaining effect... fnb shenandoah

Frontiers Rapid Enrichment and Isolation of …

WebPure DAPI-DNA complexes fluorescence is blue-white, while pure DAPI-PolyP com-plexes fluorescence is yellow [1,12]. DAPI-lipid fluo-rescence is white-yellow, week and fades in a few sec-onds [12]. In living cells mixed fluorescence spectra may be observed [13,14]. The green fluorescence of DAPI appears if PolyP are co-localyzed with DNA, or if WebNov 13, 2007 · Polyphosphate (polyP) consists of tens to hundreds of phosphates, linked by ATP-like high-energy bonds. Although polyP is present in mammalian mitochondria, its physiological roles there are obscure. Here, we examine the involvement of polyP in mitochondrial energy metabolism and ion transport. WebA novel fluorescence PPK1 enzymatic assay was developed using DAPI. The fluorescence assay was used for screening of polyP synthesis inhibitors. The assay will be useful in high-throughput screening of putative antivirulence molecules targeting enteric pathogens. Acknowledgments fnbshawnee.com