WebSep 29, 2011 · Plates were then sealed with Airpore tape (Qiagen, Cat. No. 19571) and incubated for 20 hours at 30°C in a HiGro shaker-incubator (GeneMachines, Genomic Solutions, San Carlos, CA). GFP fluorescence (excitation 485 nm, emission 528 nm) and cell density by OD at 600 nm were measured using a plate reader Analyst GT (Molecular … Webthe HiGro can facilitate significant DNA plasmid yield after a very short growth period. The combination of convenience, high-capacity and unique growth controls makes the HiGro shaker an ideal solution for high-throughput, plasmid growth applications. Table 1. …
Lab Shakers Thermo Fisher Scientific - US
WebFeb 1, 2005 · These collections consist of strains with an epitope tag integrated at the C-terminus of essentially every open reading frame (ORF), one with the tandem affinity purification (TAP) tag, and one... WebFeb 5, 2009 · Putative transposants were picked robotically using a Flexsys colony picker (Genomic Solutions) into 40 μl of BHI broth containing colistin, ampicillin, and gentamicin in 384-well microtiter plates (Fisher Scientific) and grown overnight in a HiGro shaker (Genomic Solutions). der dutchman sarasota facebook
3000W Micro Hydroelectric Generator Hydro Power Station Water …
WebFeb 6, 2007 · The clones were grown at 37°C in a HiGro shaker (Genomic Solutions) for 24 hours. Wells E7 and H12 were inoculated with the same BAC clone on all plates to serve as internal controls for plate orientation and fingerprinting quality. The DNA was isolated via an alkaline lysis method with Qiagen reagents (Qiagen, Inc., Valencia, CA) in a 96-well ... WebFurthermore, the HiGro shaker conditions for this baseline protocol were adapted with few changes from those conditions used for standard plasmid amplification in an E. coli host. With minimal effort, we have been able to determine a working protocol for protein … Web- Shaker Speed: Maximum 550 to 650 RPM, control resolution 1 RPM - Orbit: 8.0 mm (96-well format), 4.0 mm (384-well format)mat), 4.0 mm (384-well format) If for any reason you are not satisfied with order, please contact us directly BEFORE opening a case or contacting . We will make every effort to resolve any issues that may arise in a timely ... dere 10th